Jump to main content
Jump to site search

Issue 6, 2009
Previous Article Next Article

Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-α induced apoptosis

Author affiliations

Abstract

Fluorescence lifetime images of HeLa cells expressing enhanced green fluorescent protein (EGFP) have been measured as apoptosis is induced by tumor necrosis factor-α (TNF-α) in combination with cycloheximide. The fluorescence lifetime of EGFP is found to decrease after the induction of apoptosis, indicating that the change in environment occurs around the chromophore of EGFP with the apoptosis process. The fluorescence lifetime imaging technique can be used to perform in vivo observation of cell death processes. Fluorescence lifetime measurements are useful to examine the induction of the apoptosis process, even when a morphological change of each cell cannot be observed because of a low spatial resolution.

Graphical abstract: Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-α induced apoptosis

Back to tab navigation

Article information


Submitted
03 Feb 2009
Accepted
17 Mar 2009
First published
14 Apr 2009

Photochem. Photobiol. Sci., 2009,8, 763-767
Article type
Paper

Fluorescence lifetime images of green fluorescent protein in HeLa cells during TNF-α induced apoptosis

T. Ito, S. Oshita, T. Nakabayashi, F. Sun, M. Kinjo and N. Ohta, Photochem. Photobiol. Sci., 2009, 8, 763
DOI: 10.1039/B902341K

Search articles by author

Spotlight

Advertisements