Issue 6, 2007

Recognition of various biomolecules by the environment-sensitive spectral responses of hypocrellin B

Abstract

In this work, the spectral responses of hypocrellin B (HB) to the microenvironments of various biomolecules were studied, with human serum albumin (HSA), bovine serum albumin (BSA) and ovalbumin (OVA) used as the models for proteins, sodium alginate (SOA) and hyaluronan (HYA) for polysaccharides and liposomes for lipid membranes. Generally, compared to those in aqueous solution, the absorbance and fluorescence of HB were all strengthened in the model systems except for the fluorescence in HYA. Specially, according to the spectral responses of HB, the microenvironments in biomolecules and liposomes could be set in a sequence of hydrophobic grades, i.e., liposomes > proteins > polysaccharides. Further, RF/A, a parameter defined as the ratio of the fluorescence intensity to the absorbance, was proposed to identify the microenvironment quantitatively. It was found that the RF/A could not only distinguish various types of biomolecules but also identify specific binding from nonspecific binding to proteins or polysaccharides.

Graphical abstract: Recognition of various biomolecules by the environment-sensitive spectral responses of hypocrellin B

Article information

Article type
Paper
Submitted
21 Dec 2006
Accepted
27 Mar 2007
First published
18 Apr 2007

Photochem. Photobiol. Sci., 2007,6, 683-688

Recognition of various biomolecules by the environment-sensitive spectral responses of hypocrellin B

L. Song, J. Xie, C. Zhang, C. Li and J. Zhao, Photochem. Photobiol. Sci., 2007, 6, 683 DOI: 10.1039/B618678E

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