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Issue 4, 2006
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Reaction mechanisms of riboflavin triplet state with nucleic acid bases

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Abstract

ESR and laser flash photolysis studies have determined a reasonable order of reactivity of nucleotides with triplet riboflavin (3Rb*) for the first time. ESR detection of triplet state reactivity of Rb with nucleoside, polynucleotide and DNA has been obtained simultaneously. In addition, ESR spin elimination measurement of the reactivity of 3Rb* with nucleotides in good accord with laser flash photolysis determination of the corresponding rate constants offers a simple and reliable method to detect the reactivities of nucleic acids and its components with photoexcited flavins. Kinetic, ESR and thermodynamic studies have demonstrated that Rb should be a strong endogenous photosensitizer capable of oxidizing all nucleic acid bases, and preferentially two purine nucleotides with high rate constants.

Graphical abstract: Reaction mechanisms of riboflavin triplet state with nucleic acid bases

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Article information


Submitted
18 Nov 2005
Accepted
23 Feb 2006
First published
09 Mar 2006

Photochem. Photobiol. Sci., 2006,5, 422-425
Article type
Paper

Reaction mechanisms of riboflavin triplet state with nucleic acid bases

W. Lin, C. Lu, F. Du, Z. Shao, Z. Han, T. Tu, S. Yao and N. Lin, Photochem. Photobiol. Sci., 2006, 5, 422
DOI: 10.1039/B516424A

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