Enzymatic in vitro reduction of ketones. Part 8. A new model for the reduction of cyclic ketones by horse liver alcohol dehydrogenase. (HLAD)

Abstract

The values for the reaction rate constants of the catalytic step HLAD-NADH + ketone → HLAD-NAD⁺+ alcohol in the HLAD-catalysed reduction of cyclic ketones are rationalized in a new model. The fundamental concepts of the model are discussed. An extensive description of the model-building is presented and its predictive value for any possible six-membered ring substrate is shown. A comparison with previous models is given.