Polarographic reduction of a pyridoxal 5′-phosphate Schiff's base
Abstract
A Schiff's base of pyridoxal 5′-phosphate formed by reaction with n-hexylamine was reduced on a dropping mercury electrode. The polarographic reduction was studied as a function of pH and solvent composition in NN-dimethylformamide–water mixtures. Uptake of two protons before the rate-limiting step was observed in all cases.