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Issue 40, 2020
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l-DNA-tagged fluorescence in situ hybridization for highly sensitive imaging of RNAs in single cells

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Abstract

We report an effective fluorescence in situ hybridization strategy, named L-DNA tagged FISH (LT-FISH), for highly sensitive RNA detection in fixed cultured cells. LT-FISH includes two-step hybridization processes with a LD chimera oligonucleotide probe and a fluorescence-labeled PCR product tethering a L-DNA tag. The degree of fluorescence enhancement, depending on the length of PCR products, was up to 14-fold when the 606 bp product was used. Endogenous mRNA and miRNA in cancer cells were visualized by utilizing this L-DNA-mediated signal amplification technique.

Graphical abstract: l-DNA-tagged fluorescence in situ hybridization for highly sensitive imaging of RNAs in single cells

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Supplementary files

Article information


Submitted
07 Aug 2020
Accepted
22 Sep 2020
First published
22 Sep 2020

Org. Biomol. Chem., 2020,18, 8084-8088
Article type
Communication

L-DNA-tagged fluorescence in situ hybridization for highly sensitive imaging of RNAs in single cells

M. Ogata, G. Hayashi, A. Ichiu and A. Okamoto, Org. Biomol. Chem., 2020, 18, 8084
DOI: 10.1039/D0OB01635G

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