Disulphide-mediated site-directed modification of proteins†
Methods for chemical modification of native proteins in a controlled fashion are in high demand. Here, a novel protocol that exploits bifunctional reagents for transient targeting of solvent exposed disulphides to direct the introduction of a single exogenous reactive thiol handle at a lysine side chain has been developed. The protocol has successfully been applied to functionalize six different Fabs and human growth hormone.
- This article is part of the themed collections: Editor’s Collection, Organic & Biomolecular Chemistry HOT article collection, Methodology development for protein modifications and Chemical biology in OBC