Issue 46, 2019
From the journal:

Organic & Biomolecular Chemistry

Highly sensitive quantification of site-specific 5-hydroxymethylcytosine at single-base resolution by HpaII-mediated ligation PCR†

Zhenhao Zhang,a   Xiao Shan,a   Pengbo Zhang,b   Weiliang Liu,a   Jingli Yan*a  and  Zhengping Li ORCID logo *a  

* Corresponding authors

a Key laboratory of Applied Surface and Colloid Chemistry Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China
E-mail: lzpbd@snnu.edu.cn, yanjingli@snnu.edu.cn

b School of Chemistry and Biology Engineering, University of Science and Technology Beijing, Beijing, P. R. China

Abstract

A highly sensitive HpaII-mediated ligation PCR assay that can precisely discriminate 5hmC from 5mC and C is developed for the quantitative determination of site-specific 5hmC in genomic DNA samples.

Graphical abstract: Highly sensitive quantification of site-specific 5-hydroxymethylcytosine at single-base resolution by HpaII-mediated ligation PCR

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Article information

DOI
https://doi.org/10.1039/C9OB02429H
Article type
Communication
Submitted
11 Nov 2019
Accepted
12 Nov 2019
First published
13 Nov 2019

Org. Biomol. Chem., 2019,17, 9849-9853

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Highly sensitive quantification of site-specific 5-hydroxymethylcytosine at single-base resolution by HpaII-mediated ligation PCR

Z. Zhang, X. Shan, P. Zhang, W. Liu, J. Yan and Z. Li, Org. Biomol. Chem., 2019, 17, 9849 DOI: 10.1039/C9OB02429H

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