Issue 11, 2019

A parallel permeability assay of peptides across artificial membranes and cell monolayers using a fluorogenic reaction

Abstract

Here, we report a facile permeability assay to quantitatively evaluate the membrane permeability of multiple peptides in parallel. With a fluorogenic click reaction between azidocoumarin and a terminal alkyne tag introduced on a peptide, the peptide that crossed an artificial membrane or a cell monolayer was quantitatively detected. The method allows a rapid measurement of the permeability of multiple compounds on a plate reader even in the presence of a complex mixture of biological molecules.

Graphical abstract: A parallel permeability assay of peptides across artificial membranes and cell monolayers using a fluorogenic reaction

Supplementary files

Article information

Article type
Communication
Submitted
17 Jan 2019
Accepted
19 Feb 2019
First published
21 Feb 2019

Org. Biomol. Chem., 2019,17, 2887-2891

Author version available

A parallel permeability assay of peptides across artificial membranes and cell monolayers using a fluorogenic reaction

J. Morimoto, R. Amano, T. Ono and S. Sando, Org. Biomol. Chem., 2019, 17, 2887 DOI: 10.1039/C9OB00133F

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