Heterologous production of asperipin-2a: proposal for sequential oxidative macrocyclization by a fungi-specific DUF3328 oxidase

Ying Ye; Taro Ozaki; Myco Umemura; Chengwei Liu; Atsushi Minami; Hideaki Oikawa

doi:10.1039/C8OB02824A

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Heterologous production of asperipin-2a: proposal for sequential oxidative macrocyclization by a fungi-specific DUF3328 oxidase†

Ying Ye,^a Taro Ozaki,

*^a Myco Umemura,^bc Chengwei Liu,^a Atsushi Minami^a and Hideaki Oikawa

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060-0810, Hokkaido, Japan
E-mail: ozaki@sci.hokudai.ac.jp, hoik@sci.hokudai.ac.jp

^b Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Ibaraki, Japan

^c Computational Bio Big Data Open Innovation Laboratory (CBBD-OIL), AIST, Tsukuba 305-8566, Ibaraki, Japan

Abstract

Asperipin-2a is a ribosomally synthesized and post-translationally modified peptide isolated from Asperigillus flavus. Herein, we report the heterologous production of asperipin-2a and determination of its absolute structure. Notably, the characteristic bicyclic structure was likely constructed by a single oxidase containing the DUF3328 domain.

This article is part of the themed collections: Chemical Biology in OBC and Biosynthesis

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Article information

DOI: https://doi.org/10.1039/C8OB02824A
Article type: Communication
Submitted: 13 Nov 2018
Accepted: 28 Nov 2018
First published: 28 Nov 2018

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Org. Biomol. Chem., 2019,17, 39-43

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Heterologous production of asperipin-2a: proposal for sequential oxidative macrocyclization by a fungi-specific DUF3328 oxidase

Y. Ye, T. Ozaki, M. Umemura, C. Liu, A. Minami and H. Oikawa, Org. Biomol. Chem., 2019, 17, 39 DOI: 10.1039/C8OB02824A

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Organic & Biomolecular Chemistry

Heterologous production of asperipin-2a: proposal for sequential oxidative macrocyclization by a fungi-specific DUF3328 oxidase†

Abstract

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Heterologous production of asperipin-2a: proposal for sequential oxidative macrocyclization by a fungi-specific DUF3328 oxidase

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