Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 34, 2018
Previous Article Next Article

An off–on fluorescent probe for the detection of mitochondria-specific protein persulfidation

Author affiliations

Abstract

Protein persulfidation is a newly defined oxidative posttranslational modification and plays important roles in many biological processes. Detection of protein persulfidation in living systems is urgently needed to advance the study of H2S/H2Sn-based signalling and cellular redox regulation. Here, we developed a novel off–on fluorescent probe for the detection of persulfidation using a chemical sensor, HQO-SSH, in biological systems. HQO-SSH features fast reaction, good selectivity and high sensitivity. Due to the distinctive features of HQO-SSH, this probe was successfully applied to image protein persulfidation changes in pulmonary cells. We also demonstrated that the probe is suitable for imaging protein persulfidation in lung tissues. In addition, confocal imaging with this method revealed that sulfur mustard, a commonly used chemical warfare agent, decreased mitochondrial protein persulfidation in living lung cells and tissues. Due to these results, this probe holds great promise for exploring the role of protein persulfidation in a variety of pathophysiological conditions.

Graphical abstract: An off–on fluorescent probe for the detection of mitochondria-specific protein persulfidation

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 Jul 2018, accepted on 13 Aug 2018 and first published on 18 Aug 2018


Article type: Paper
DOI: 10.1039/C8OB01608A
Citation: Org. Biomol. Chem., 2018,16, 6350-6357

  •   Request permissions

    An off–on fluorescent probe for the detection of mitochondria-specific protein persulfidation

    W. Meng, Y. Chen, Y. Feng, H. Zhang, Q. Xu, M. Sun, W. Shi, J. Cen, J. Zhao and K. Xiao, Org. Biomol. Chem., 2018, 16, 6350
    DOI: 10.1039/C8OB01608A

Search articles by author

Spotlight

Advertisements