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Issue 37, 2018
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Synthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometry

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Abstract

Two novel cyclic quaternary amine crosslinking probes are synthesized for structural mass spectrometry of protein complexes in solution and for analysis of protein interactions in organellar and whole cell extracts. Each exhibits high aqueous solubility, excellent protein crosslinking efficiencies, low collision induced dissociation (CID) energy fragmentation efficiencies, high stoichiometries of reaction, increased charges of crosslinked peptide ions, and maintenance of overall surface charge balance of crosslinked proteins.

Graphical abstract: Synthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometry

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Supplementary files

Article information


Submitted
08 Feb 2018
Accepted
07 Mar 2018
First published
07 Mar 2018

Org. Biomol. Chem., 2018,16, 8245-8248
Article type
Communication

Synthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometry

S. E. Hagen, K. Liu, Y. Jin, L. Piersimoni, P. C. Andrews and H. D. Showalter, Org. Biomol. Chem., 2018, 16, 8245
DOI: 10.1039/C8OB00329G

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