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Issue 37, 2018
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Synthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometry

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Abstract

Two novel cyclic quaternary amine crosslinking probes are synthesized for structural mass spectrometry of protein complexes in solution and for analysis of protein interactions in organellar and whole cell extracts. Each exhibits high aqueous solubility, excellent protein crosslinking efficiencies, low collision induced dissociation (CID) energy fragmentation efficiencies, high stoichiometries of reaction, increased charges of crosslinked peptide ions, and maintenance of overall surface charge balance of crosslinked proteins.

Graphical abstract: Synthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometry

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Publication details

The article was received on 08 Feb 2018, accepted on 07 Mar 2018 and first published on 07 Mar 2018


Article type: Communication
DOI: 10.1039/C8OB00329G
Citation: Org. Biomol. Chem., 2018,16, 8245-8248

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    Synthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometry

    S. E. Hagen, K. Liu, Y. Jin, L. Piersimoni, P. C. Andrews and H. D. Showalter, Org. Biomol. Chem., 2018, 16, 8245
    DOI: 10.1039/C8OB00329G

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