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Issue 39, 2016
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Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants

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Abstract

A transaminase from Halomonas elongata and four mutants generated by an in silico-based design were recombinantly produced in E. coli, purified and applied to the amination of mono-substituted aromatic carbonyl-derivatives. While benzaldehyde derivatives were excellent substrates, only NO2-acetophenones were transformed into the (S)-amine with a high enantioselectivity. The different behaviour of wild-type and mutated transaminases was assessed by in silico substrate binding mode studies.

Graphical abstract: Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants

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Article information


Submitted
29 Jul 2016
Accepted
06 Sep 2016
First published
06 Sep 2016

Org. Biomol. Chem., 2016,14, 9306-9311
Article type
Paper

Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants

M. L. Contente, M. Planchestainer, F. Molinari and F. Paradisi, Org. Biomol. Chem., 2016, 14, 9306
DOI: 10.1039/C6OB01629D

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