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Issue 35, 2016
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Single dish gradient screening of small molecule localization

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Abstract

Understanding trafficking in cells and tissues is one of the most critical steps in exploring the mechanisms and modes of action (MOAs) of a small molecule. Typically, deciphering the role of concentration presents one of the most difficult challenges associated with this task. Herein, we present a practical solution to this problem by developing concentration gradients within single dishes of cells. We demonstrate the method by evaluating fluorescently-labelled probes developed from two classes of natural products that have been identified as potential anti-cancer leads by STORM super-resolution microscopy.

Graphical abstract: Single dish gradient screening of small molecule localization

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Supplementary files

Article information


Submitted
01 Jul 2016
Accepted
10 Aug 2016
First published
17 Aug 2016

Org. Biomol. Chem., 2016,14, 8241-8245
Article type
Communication
Author version available

Single dish gradient screening of small molecule localization

P. Beuzer, J. Axelrod, L. Trzoss, W. Fenical, R. Dasari, A. Evidente, A. Kornienko, H. Cang and J. J. La Clair, Org. Biomol. Chem., 2016, 14, 8241
DOI: 10.1039/C6OB01418F

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