Issue 44, 2014
From the journal:

Organic & Biomolecular Chemistry

Templated DNA ligation with thiol chemistry†

Dadong Li,‡a   Xiaojian Wang,‡a   Fubo Shi,‡a   Ruojie Sha,a   Nadrian C. Seeman*a  and  James W. Canary*a  

* Corresponding authors

a Department of Chemistry, New York University, 100 Washington Sq. East, New York, NY 10003, USA
E-mail: james.canary@nyu.edu, ned.seeman@nyu.edu

Abstract

We describe two DNA-templated ligation strategies: native chemical ligation (NCL), and thiol-disulfide exchange. Both systems result in successful ligation in the presence of a DNA template. The stability of the product from the NCL reaction relies on exogenous thiol, while the thiol-disulfide reaction proceeds in a catalyst-free manner.

Graphical abstract: Templated DNA ligation with thiol chemistry

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Article information

DOI
https://doi.org/10.1039/C4OB01552E
Article type
Communication
Submitted
23 Jul 2014
Accepted
24 Sep 2014
First published
06 Oct 2014

Org. Biomol. Chem., 2014,12, 8823-8827

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Templated DNA ligation with thiol chemistry

D. Li, X. Wang, F. Shi, R. Sha, N. C. Seeman and J. W. Canary, Org. Biomol. Chem., 2014, 12, 8823 DOI: 10.1039/C4OB01552E

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