Jump to main content
Jump to site search

Issue 17, 2013
Previous Article Next Article

Enzyme-mediated nutrient release: glucose-precursor activation by β-galactosidase to induce bacterial growth

Author affiliations

Abstract

Bacteria will gain an advantage if they are able to metabolize nutrients that are inaccessible for other bacteria. To demonstrate this principle, we developed a simple model system, which mimics how bacteria exploit natural carbon sources. A masked glucose precursor that is activated by β-galactosidase was used as a carbon source for bacterial growth in a glucose-deficient medium. No bacterial growth was observed in the presence of control substances in which β-galactosidase mediated cleavage did not lead to glucose release. This study represents a proof-of-principle example in which a bacterium can grow in a nutrient-free medium by inducible, enzyme-mediated nutrient release from a precursor.

Graphical abstract: Enzyme-mediated nutrient release: glucose-precursor activation by β-galactosidase to induce bacterial growth

Back to tab navigation

Article information


Submitted
09 Dec 2012
Accepted
24 Feb 2013
First published
26 Feb 2013

Org. Biomol. Chem., 2013,11, 2903-2910
Article type
Paper

Enzyme-mediated nutrient release: glucose-precursor activation by β-galactosidase to induce bacterial growth

N. Karton-Lifshin, U. Vogel, E. Sella, P. H. Seeberger, D. Shabat and B. Lepenies, Org. Biomol. Chem., 2013, 11, 2903
DOI: 10.1039/C3OB27385G

Social activity

Search articles by author

Spotlight

Advertisements