Metal free, “click and click–click” conjugation of ribonucleosides and 2′-OMe oligoribonucleotides on the solid phase

Abstract

A fast and practical metal free conjugation of ribonucleosides and 2′-OMe 4-mer oligoribonucleotides has been accomplished by a nitrile oxide alkyne click cycloaddition reaction on the solid-phase, the methodology is suited to modification at either, or both, the 3′- or the 5′-terminus of the oligoribonucleotide substrate.