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Issue 3, 2010
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Synthetic arabinomannan glycolipids and their effects on growth and motility of the Mycobacterium smegmatis

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Abstract

Arabinomannan-containing glycolipids, relevant to the mycobacterial cell-wall component lipoarabinomannan, were synthesized by chemical methods. The glycolipids were presented with tri- and tetrasaccharide arabinomannans as the sugar portion and a double alkyl chain as the lyophilic portion. Following synthesis, systematic biological and biophysical studies were undertaken in order to identify the effects of the glycolipids during mycobacterium growth. The studies included mycobacterial growth, biofilm formation and motility assays. From the studies, it was observed that the synthetic glycolipid with higher arabinan residues inhibited the mycobacterial growth, lessened the biofilm formation and impaired the motility of mycobacteria. A surface plasmon resonance study involving the immobilized glycan surface and the mycobacterial crude lysates as analytes showed specificities of the interactions. Further, it was found that cell lysates from motile bacteria bound oligosaccharide with higher affinity than non-motile bacteria.

Graphical abstract: Synthetic arabinomannan glycolipids and their effects on growth and motility of the Mycobacterium smegmatis

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Publication details

The article was received on 18 Aug 2009, accepted on 22 Oct 2009 and first published on 02 Dec 2009


Article type: Paper
DOI: 10.1039/B917070G
Org. Biomol. Chem., 2010,8, 592-599

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    Synthetic arabinomannan glycolipids and their effects on growth and motility of the Mycobacterium smegmatis

    K. Naresh, B. K. Bharati, P. G. Avaji, N. Jayaraman and D. Chatterji, Org. Biomol. Chem., 2010, 8, 592
    DOI: 10.1039/B917070G

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