SCHEDULED MAINTENANCE
We report a simple and rapid method for quantitation of single-to-double stranded (ss ∶ ds) DNA ratios in solution, using steady-state measurements of fluorescence from two simultaneously excited intercalated dyes; the ratio of fluorescence intensities from PicoGreen (525 nm) and ethidium bromide (610 nm) is directly proportional to the ss ∶ ds DNA ratio.
L. Beach, C. Schweitzer and J. C. Scaiano, Org. Biomol. Chem., 2003, 1, 450 DOI: 10.1039/B209284K
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