Label-free fluorescent immunoassay for Cu2+ ion detection based on UV degradation of immunocomplex and metal ion chelates

Qi Shu; Mengli Liu; Hui Ouyang; Zhifeng Fu

doi:10.1039/C7NR04087C

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Label-free fluorescent immunoassay for Cu²⁺ ion detection based on UV degradation of immunocomplex and metal ion chelates†

Qi Shu,^a Mengli Liu,^a Hui Ouyang^a and Zhifeng Fu

*^a

Author affiliations

* Corresponding authors

^a Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China
E-mail: fuzf@swu.edu.cn
Fax: +86-23-68251048
Tel: +86-23-68250184

Abstract

A mouse anti Cu²⁺-EDTA monoclonal antibody was used to capture EDTA chelated Cu²⁺ ions. Then UV radiation was utilized to degrade immunocomplex and Cu²⁺-EDTA chelates to release free Cu²⁺ ions. Based on their quenching effect to the fluorescent emission of CdSe/ZnS quantum dots, the released Cu²⁺ ions could be detected with ideal specificity and high sensitivity.

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Article information

DOI: https://doi.org/10.1039/C7NR04087C
Article type: Communication
Submitted: 08 Jun 2017
Accepted: 30 Jul 2017
First published: 01 Aug 2017

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Nanoscale, 2017,9, 12302-12306

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Label-free fluorescent immunoassay for Cu²⁺ ion detection based on UV degradation of immunocomplex and metal ion chelates

Q. Shu, M. Liu, H. Ouyang and Z. Fu, Nanoscale, 2017, 9, 12302 DOI: 10.1039/C7NR04087C

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