Jump to main content
Jump to site search

Issue 27, 2017
Previous Article Next Article

Enzymatic biomineralization of biocompatible CuInS2, (CuInZn)S2 and CuInS2/ZnS core/shell nanocrystals for bioimaging

Author affiliations

Abstract

This work demonstrates a bioenabled fully aqueous phase and room temperature route to the synthesis of CuInS2/ZnS core/shell quantum confined nanocrystals conjugated to IgG antibodies and used for fluorescent tagging of THP-1 leukemia cells. This elegant, straightforward and green approach avoids the use of solvents, high temperatures and the necessity to phase transfer the nanocrystals prior to application. Non-toxic CuInS2, (CuInZn)S2, and CuInS2/ZnS core/shell quantum confined nanocrystals are synthesized via a biomineralization process based on a single recombinant cystathionine γ-lyase (CSE) enzyme. First, soluble In–S complexes are formed from indium acetate and H2S generated by CSE, which are then stabilized by L-cysteine in solution. The subsequent addition of copper, or both copper and zinc, precursors then results in the immediate formation of CuInS2 or (CuInZn)S2 quantum dots. Shell growth is realized through subsequent introduction of Zn acetate to the preformed core nanocrystals. The size and optical properties of the nanocrystals are tuned by adjusting the indium precursor concentration and initial incubation period. CuInS2/ZnS core/shell particles are conjugated to IgG antibodies using EDC/NHS cross-linkers and then applied in the bioimaging of THP-1 cells. Cytotoxicity tests confirm that CuInS2/ZnS core/shell quantum dots do not cause cell death during bioimaging. Thus, this biomineralization enabled approach provides a facile, low temperature route for the fully aqueous synthesis of non-toxic CuInS2/ZnS quantum dots, which are ideal for use in bioimaging applications.

Graphical abstract: Enzymatic biomineralization of biocompatible CuInS2, (CuInZn)S2 and CuInS2/ZnS core/shell nanocrystals for bioimaging

Back to tab navigation

Supplementary files

Publication details

The article was received on 21 Apr 2017, accepted on 22 Jun 2017 and first published on 23 Jun 2017


Article type: Paper
DOI: 10.1039/C7NR02852K
Nanoscale, 2017,9, 9340-9351

  •   Request permissions

    Enzymatic biomineralization of biocompatible CuInS2, (CuInZn)S2 and CuInS2/ZnS core/shell nanocrystals for bioimaging

    L. C. Spangler, R. Chu, L. Lu, C. J. Kiely, B. W. Berger and S. McIntosh, Nanoscale, 2017, 9, 9340
    DOI: 10.1039/C7NR02852K

Search articles by author

Spotlight

Advertisements