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Issue 4, 2017
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Preparation and application of silver nanoparticle-functionalized magnetic graphene oxide nanocomposites

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Abstract

Although selective enrichment of glycopeptides from complex biological samples is indispensable for mass spectrometry (MS)-based glycoproteomics, it still remains a great challenge due to the low abundance of glycoproteins and suppression of non-glycopeptides. In this study, silver nanoparticle-functionalized magnetic graphene oxide nanocomposites (GO/Fe3O4/PEI/Ag) were synthesized. Silver nanoparticles were generated in situ on the surface of magnetic graphene oxide using polyethylenimine as a reducing and stabilizing agent. The resulting material was used as an adsorbent for selective enrichment of glycopeptides. GO/Fe3O4/PEI/Ag nanocomposites offered excellent enrichment ability, which was attributed to the synergistic effect of polyethylenimine and silver nanoparticles. The nanocomposites showed superior specificity for glycopeptides even when non-glycopeptides were 100 times more concentrated than glycopeptides. The nanocomposites displayed advantages including rapid adsorption (1 min), low detection limit (25 fmol), repeatability (6 times), and high recovery (77.8%). Using these nanocomposites, 91 different glycoproteins and 136 N-linked glycopeptides were identified from among 20 μg tryptic human serum proteins and this demonstrated the superior performance of the nanocomposites for glycopeptides enrichment.

Graphical abstract: Preparation and application of silver nanoparticle-functionalized magnetic graphene oxide nanocomposites

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Supplementary files

Article information


Submitted
29 Nov 2016
Accepted
20 Dec 2016
First published
23 Dec 2016

Nanoscale, 2017,9, 1607-1615
Article type
Paper

Preparation and application of silver nanoparticle-functionalized magnetic graphene oxide nanocomposites

B. Jiang, Q. Wu, L. Zhang and Y. Zhang, Nanoscale, 2017, 9, 1607
DOI: 10.1039/C6NR09260H

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