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Issue 4, 2017
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Search for effective chemical quenching to arrest molecular assembly and directly monitor DNA nanostructure formation

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Abstract

Structural DNA nanotechnology has demonstrated both versatility and potential as a molecular manufacturing tool; the formation and processing of DNA nanostructures has therefore been subject to much interest. Characterization of the formation process itself is vital to understanding the role of design in production yield. We present our search for a robust new technique, chemical quenching, to arrest molecular folding in DNA systems for subsequent characterization. Toward this end we will introduce two miniM13 origami designs based on a 2.4 kb scaffold, each with diametrically opposed scaffold routing strategies (maximized scaffold crossovers versus maximized staple crossovers) to examine the relevance of design in the folding process. By chemically rendering single strand DNA inert and unable to hybridize, we probe the folding pathway of several scaffolded DNA origami structures.

Graphical abstract: Search for effective chemical quenching to arrest molecular assembly and directly monitor DNA nanostructure formation

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Supplementary files

Article information


Submitted
27 Oct 2016
Accepted
25 Dec 2016
First published
26 Dec 2016

Nanoscale, 2017,9, 1637-1644
Article type
Paper

Search for effective chemical quenching to arrest molecular assembly and directly monitor DNA nanostructure formation

J. M. Majikes, J. A. Nash and T. H. LaBean, Nanoscale, 2017, 9, 1637
DOI: 10.1039/C6NR08433H

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