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Issue 1, 2017
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Mechanism of α-synuclein translocation through a VDAC nanopore revealed by energy landscape modeling of escape time distributions

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Abstract

We probe the energy landscape governing the passage of α-synuclein, a natural “diblock copolymer”-like polypeptide, through a nanoscale pore. α-Synuclein is an intrinsically disordered neuronal protein associated with Parkinson's pathology. The motion of this electrically heterogeneous polymer in the β-barrel voltage-dependent anion channel (VDAC) of the mitochondrial outer membrane strongly depends on the properties of both the charged and uncharged regions of the α-synuclein polymer. We model this motion in two ways. First, a simple Markov model accounts for the transitions of the channel between the states of different occupancy by α-synuclein. Second, the detailed energy landscape of this motion can be accounted for using a drift-diffusion framework that incorporates the α-synuclein binding energy and the free energy cost of its confinement in the VDAC pore. The models directly predict the probability of α-synuclein translocation across the mitochondrial outer membrane, with immediate implications for the physiological role of α-synuclein in regulation of mitochondrial bioenergetics. Time-resolved measurements of the electrical properties of VDAC occupied by α-synuclein reveal distinct effects of the motion of the junction separating the differently charged regions of the polymer.

Graphical abstract: Mechanism of α-synuclein translocation through a VDAC nanopore revealed by energy landscape modeling of escape time distributions

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Article information


Submitted
17 Oct 2016
Accepted
17 Nov 2016
First published
30 Nov 2016

Nanoscale, 2017,9, 183-192
Article type
Paper

Mechanism of α-synuclein translocation through a VDAC nanopore revealed by energy landscape modeling of escape time distributions

D. P. Hoogerheide, P. A. Gurnev, T. K. Rostovtseva and S. M. Bezrukov, Nanoscale, 2017, 9, 183 DOI: 10.1039/C6NR08145B

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