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Issue 22, 2016
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Probing protein complexes inside living cells using a silicon nanowire-based pull-down assay

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Abstract

Most proteins perform their functions as interacting complexes. Here we propose a novel method for capturing an intracellular protein and its interacting partner out of living cells by utilizing intracellular access of antibody modified vertical silicon nanowire arrays whose surface is covered with a polyethylene glycol layer to prevent strong cell adhesion. Such a feature facilitates the removal of cells by simple washing, enabling subsequent detection of a pulled-down protein and its interacting partner, and further assessment of a drug-induced change in the interacting complex. Our new SiNW-based tool is thus suitable for authentication of protein networks inside living cells.

Graphical abstract: Probing protein complexes inside living cells using a silicon nanowire-based pull-down assay

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Publication details

The article was received on 08 Jan 2016, accepted on 06 May 2016 and first published on 06 May 2016


Article type: Communication
DOI: 10.1039/C6NR00171H
Citation: Nanoscale, 2016,8, 11380-11384

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    Probing protein complexes inside living cells using a silicon nanowire-based pull-down assay

    S. Choi, H. Kim, S. Y. Kim and E. G. Yang, Nanoscale, 2016, 8, 11380
    DOI: 10.1039/C6NR00171H

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