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Issue 41, 2015

Quantitative nanoscale electrostatics of viruses

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Abstract

Electrostatics is one of the fundamental driving forces of the interaction between biomolecules in solution. In particular, the recognition events between viruses and host cells are dominated by both specific and non-specific interactions and the electric charge of viral particles determines the electrostatic force component of the latter. Here we probe the charge of individual viruses in liquid milieu by measuring the electrostatic force between a viral particle and the Atomic Force Microscope tip. The force spectroscopy data of co-adsorbed ϕ29 bacteriophage proheads and mature virions, adenovirus and minute virus of mice capsids is utilized for obtaining the corresponding density of charge for each virus. The systematic differences of the density of charge between the viral particles are consistent with the theoretical predictions obtained from X-ray structural data. Our results show that the density of charge is a distinguishing characteristic of each virus, depending crucially on the nature of the viral capsid and the presence/absence of the genetic material.

Graphical abstract: Quantitative nanoscale electrostatics of viruses

Supplementary files

Article information


Submitted
26 Jun 2015
Accepted
16 Jul 2015
First published
22 Jul 2015

Nanoscale, 2015,7, 17289-17298
Article type
Paper
Author version available

Quantitative nanoscale electrostatics of viruses

M. Hernando-Pérez, A. X. Cartagena-Rivera, A. Lošdorfer Božič, P. J. P. Carrillo, C. San Martín, M. G. Mateu, A. Raman, R. Podgornik and P. J. de Pablo, Nanoscale, 2015, 7, 17289 DOI: 10.1039/C5NR04274G

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