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Issue 22, 2012
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Amidase-responsive controlled release of antitumoral drug into intracellular media using gluconamide-capped mesoporous silicananoparticles

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Abstract

MCM-41 silica nanoparticles were used as inorganic scaffolding to prepare a nanoscopic-capped hybrid material S1, which was able to release an entrapped cargo in the presence of certain enzymes, whereas in the absence of enzymes, a zero release system was obtained. S1 was prepared by loading nanoparticles with Safranine O dye and was then capped with a gluconamide derivative. In the absence of enzymes, the release of the dye from the aqueous suspensions of S1 was inhibited as a result of the steric hindrance imposed by the bulky gluconamide derivative, the polymerized gluconamide layer and the formation of a dense hydrogen-bonded network around the pore outlets. Upon the addition of amidase and pronase enzymes, delivery of Safranine O dye was observed due to the enzymatic hydrolysis of the amide bond in the anchored gluconamide derivative. S1 nanoparticles were not toxic for cells, as demonstrated by cell viability assays using HeLa and MCF-7 cell lines, and were associated with lysosomes, as shown by confocal microscopy. Finally, the S1–CPT material loaded with the cytotoxic drug camptothecin and capped with the gluconamide derivative was prepared. The HeLa cells treated with S1–CPT underwent cell death as a result of material internalization, and of the subsequent cellular enzyme-mediated hydrolysis and aperture of the molecular gate, which induced the release of the camptothecin cargo.

Graphical abstract: Amidase-responsive controlled release of antitumoral drug into intracellular media using gluconamide-capped mesoporous silica nanoparticles

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Article information


Submitted
30 Jul 2012
Accepted
24 Sep 2012
First published
02 Oct 2012

Nanoscale, 2012,4, 7237-7245
Article type
Paper

Amidase-responsive controlled release of antitumoral drug into intracellular media using gluconamide-capped mesoporous silica nanoparticles

I. Candel, E. Aznar, L. Mondragón, C. D. L. Torre, R. Martínez-Máñez, F. Sancenón, M. D. Marcos, P. Amorós, C. Guillem, E. Pérez-Payá, A. Costero, S. Gil and M. Parra, Nanoscale, 2012, 4, 7237
DOI: 10.1039/C2NR32062B

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