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Issue 10, 2012
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Tumor necrosis factor interaction with gold nanoparticles

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Abstract

We report on a systematic investigation of molecular conjugation of tumor necrosis factor-α (TNF) protein onto gold nanoparticles (AuNPs) and the subsequent binding behavior to its antibody (anti-TNF). We employ a combination of physical and spectroscopic characterization methods, including electrospray-differential mobility analysis, dynamic light scattering, polyacrylamide gel electrophoresis, attenuated total reflectance-Fourier transform infrared spectroscopy, fluorescence assay, and enzyme-linked immunosorbent assay. The native TNF used in this study exists in the active homotrimer configuration prior to conjugation. After binding to AuNPs, the maximum surface density of TNF is (0.09 ± 0.02) nm−2 with a binding constant of 3 × 106 (mol L−1)−1. Dodecyl sulfate ions induce desorption of monomeric TNF from the AuNP surface, indicating a relatively weak intermolecular binding within the AuNP-bound TNF trimers. Anti-TNF binds to both TNF-conjugated and citrate-stabilized AuNPs, showing that non-specific binding is significant. Based on the number of anti-TNF molecules adsorbed, a substantially higher binding affinity was observed for the TNF-conjugated surface. The inclusion of thiolated polyethylene glycol (SH–PEG) on the AuNPs inhibits the binding of anti-TNF, and the amount of inhibition is related to the number ratio of surface bound SH–PEG to TNF and the way in which the ligands are introduced. This study highlights the challenges in quantitatively characterizing complex hybrid nanoscale conjugates, and provides insight on TNF–AuNP formation and activity.

Graphical abstract: Tumor necrosis factor interaction with gold nanoparticles

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Publication details

The article was received on 21 Feb 2012, accepted on 14 Mar 2012 and first published on 22 Mar 2012


Article type: Paper
DOI: 10.1039/C2NR30415E
Nanoscale, 2012,4, 3208-3217

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    Tumor necrosis factor interaction with gold nanoparticles

    D. Tsai, S. Elzey, F. W. DelRio, A. M. Keene, K. M. Tyner, J. D. Clogston, R. I. MacCuspie, S. Guha, M. R. Zachariah and V. A. Hackley, Nanoscale, 2012, 4, 3208
    DOI: 10.1039/C2NR30415E

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