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Issue 10, 2012
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A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein

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Abstract

In this study, we report a facile method for the preparation of core–shell magnetic molecularly imprinted polymers (MIPs) for protein recognition. Uniform carboxyl group functionalized Fe3O4 nanoparticles (NPs) were synthesized using a solvothermal method. Magnetic MIPs were synthesized by self-polymerization of dopamine in the presence of template protein on the surface of the Fe3O4 NPs. A thin layer of polydopamine can be coated on Fe3O4 NPs via dopamine self-polymerization and the imprinted polydopamine shells can be controlled by the mass ratio of Fe3O4 NPs and dopamine. More importantly, there is a critical value of polydopamine shell thickness for the maximum rebinding capacity. The as-prepared lysozyme-imprinted Fe3O4@polydopamine NPs show high binding capacity and acceptable specific recognition behavior towards template proteins. This method provides the possibility for the separation and enrichment of abundant proteins in proteomic analysis.

Graphical abstract: A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein

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Article information


Submitted
10 Feb 2012
Accepted
27 Mar 2012
First published
02 Apr 2012

Nanoscale, 2012,4, 3141-3147
Article type
Paper

A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein

M. Zhang, X. Zhang, X. He, L. Chen and Y. Zhang, Nanoscale, 2012, 4, 3141
DOI: 10.1039/C2NR30316G

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