Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 21st October 2020 from 07:00 AM to 07:00 PM (BST).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 10, 2012
Previous Article Next Article

Effect of nano-hydroxyapatite on the axonal guidance growth of rat cortical neurons

Author affiliations

Abstract

Nanomaterials such as carbon nanotubes (CNT) can improve axonal connecting in a target direction during regeneration, however, it is limited by the neurotoxicity of CNT. Here we investigate the possible protective effect of nano-hydroxyapatite (n-HA) against nerve injury, as well as CNT in cultured rat cortical neurons. In this study the nanomaterials were characterized by X-Ray diffractometry (XRD) and atomic force microscopy (AFM) analysis. Our results showed that axonal migration and extension were increased significantly after n-HA treatment by immunocytochemistry assay. The patch clamp assay results showed that n-HA acts protectively after nerve injury, which inhibited the average amplitude and frequency of excitatory postsynaptic currents (EPSCs). n-HA is not neurotoxic for the electrophysiology activity of cells. To find the effect of n-HA on axonal guidance growth in the cultured cortical neurons, Netrin 1, one of the axonal guidance cues, was determined by RT-PCR and western blot assay. Compared to the control group, n-HA down-regulated the mRNA level of netrin 1, and moreover, the expression of netrin 1 decreased significantly in the cells. n-HA caused the axonal guidance growth to be mediated by netrin 1 during nerve regeneration. Therefore, the data from the present study provided a new approach for the therapy or prevention of nerve injury.

Graphical abstract: Effect of nano-hydroxyapatite on the axonal guidance growth of rat cortical neurons

Back to tab navigation

Article information


Submitted
10 Jan 2012
Accepted
19 Mar 2012
First published
21 Mar 2012

Nanoscale, 2012,4, 3201-3207
Article type
Paper

Effect of nano-hydroxyapatite on the axonal guidance growth of rat cortical neurons

M. Liu, G. Zhou, W. Song, P. Li, H. Liu, X. Niu and Y. Fan, Nanoscale, 2012, 4, 3201
DOI: 10.1039/C2NR30072A

Social activity

Search articles by author

Spotlight

Advertisements