Jump to main content
Jump to site search

Issue 3, 2012
Previous Article Next Article

Impedimetric immunoglobulin G immunosensor based on chemically modified graphenes

Author affiliations

Abstract

Immunosensors which display high sensitivity and selectivity are of utmost importance to the biomedical field. Graphene is a material which has immense potential for the fabrication of immunosensors. For the first time, we evaluate the immunosensing capabilities of various graphene surfaces in this work. We propose a simple and label-free electrochemical impedimetric immunosensor for immunoglobulin G (IgG) based on chemically modified graphene (CMG) surfaces such as graphite oxide, graphene oxide, thermally reduced graphene oxide and electrochemically reduced graphene oxide. Disposable electrochemical printed electrodes were first modified with CMG materials before anti-immunoglobulin G (anti-IgG), which is specific to IgG, was immobilized. The principle of detection lies in the changes in impedance spectra of the redox probe after the attachment of IgG to the immobilized anti-IgG. It was found that thermally reduced graphene oxide has the best performance when compared to the other CMG materials. In addition, the optimal concentration of anti-IgG to be deposited onto the modified electrode surface is 10 μg ml−1 and the linear range of detection of the immunosensor is from 0.3 μg ml−1 to 7 μg ml−1. Finally, the fabricated immunosensor also displays selectivity for IgG.

Graphical abstract: Impedimetric immunoglobulin G immunosensor based on chemically modified graphenes

Back to tab navigation

Supplementary files

Publication details

The article was received on 13 Oct 2011, accepted on 21 Nov 2011 and first published on 20 Dec 2011


Article type: Paper
DOI: 10.1039/C2NR11492E
Nanoscale, 2012,4, 921-925

  •   Request permissions

    Impedimetric immunoglobulin G immunosensor based on chemically modified graphenes

    A. H. Loo, A. Bonanni, A. Ambrosi, H. L. Poh and M. Pumera, Nanoscale, 2012, 4, 921
    DOI: 10.1039/C2NR11492E

Search articles by author

Spotlight

Advertisements