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Issue 7, 2011
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Site-specific immobilization of gold binding polypeptide on gold nanoparticle-coated graphene sheet for biosensor application

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Abstract

The effective and strong immobilization of enzymes on solid surfaces is required for current biological applications, such as microchips, biofuel cells, and biosensors. Gold-binding polypeptide (GBP), a genetically designed peptide, possesses unique and specific interactions with a gold surface, resulting in improved enzyme stability and activity. Herein we demonstrated an immobilization method for biosensor applications through site-specific interactions between GBP-fused organophosphorus hydrolase (GBP-OPH) and gold nanoparticle-coated chemically modified graphene (Au-CMG), showing enhanced sensing capability. A flow injection biosensor was fabricated by using GBP-OPH/Au-CMG to detect paraoxons, a model pesticide, showing higher sensitivity, lower detection limit and better operating stability compared that of OPH/Au-CMG. This strategy, which integrates biotic and abiotic moieties through site-specific interactions, has a great potential for use in biosensing and bioconversion process.

Graphical abstract: Site-specific immobilization of gold binding polypeptide on gold nanoparticle-coated graphene sheet for biosensor application

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Publication details

The article was received on 22 Feb 2011, accepted on 31 Mar 2011 and first published on 03 Jun 2011


Article type: Paper
DOI: 10.1039/C1NR10197H
Citation: Nanoscale, 2011,3, 2950-2956

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    Site-specific immobilization of gold binding polypeptide on gold nanoparticle-coated graphene sheet for biosensor application

    M. Yang, B. G. Choi, T. J. Park, N. S. Heo, W. H. Hong and S. Y. Lee, Nanoscale, 2011, 3, 2950
    DOI: 10.1039/C1NR10197H

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