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Issue 5, 2011
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Specific effects of surface carboxyl groups on anionic polystyrene particles in their interactions with mesenchymal stem cells

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Abstract

Nanoparticle uptake by living cells is governed by chemical interactions between functional groups on the nanoparticle as well as the receptors on cell surfaces. Here we have investigated the uptake of anionic polystyrene (PS) nanoparticles of ∼100 nm diameter by mesenchymal stem cells (MSCs) using spinning-disk confocal optical microscopy combined with a quantitative analysis of the fluorescence images. Two types of anionic PS nanoparticles with essentially identical sizes and ζ-potentials were employed in this study, carboxyl-functionalized nanoparticles (CPS) and plain PS nanoparticles, both coated with anionic detergent for stabilization. CPS nanoparticles were observed to internalize more rapidly and accumulate to a much higher level than plain PS nanoparticles. The relative importance of different uptake mechanisms for the two types of nanoparticles was investigated by using specific inhibitors. CPS nanoparticles were internalized mainly via the clathrin-mediated mechanism, whereas plain PS nanoparticles mainly utilized the macropinocytosis pathway. The pronounced difference in the internalization behavior of CPS and plain PS nanoparticles points to a specific interaction of the carboxyl group with receptors on the cell surface.

Graphical abstract: Specific effects of surface carboxyl groups on anionic polystyrene particles in their interactions with mesenchymal stem cells

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Article information


Submitted
05 Dec 2010
Accepted
18 Feb 2011
First published
15 Mar 2011

Nanoscale, 2011,3, 2028-2035
Article type
Paper

Specific effects of surface carboxyl groups on anionic polystyrene particles in their interactions with mesenchymal stem cells

X. Jiang, A. Musyanovych, C. Röcker, K. Landfester, V. Mailänder and G. U. Nienhaus, Nanoscale, 2011, 3, 2028
DOI: 10.1039/C0NR00944J

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