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Issue 5, 2016
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Photo-cross-linked small-molecule affinity matrix as a tool for target identification of bioactive small molecules

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Abstract

Covering: up to the end of 2015

A photo-cross-linked small-molecule affinity matrix is a unique platform for identifying targets for bioactive small molecules. It utilises a photogenerated carbene species to immobilise a variety of bioactive small molecules on an affinity matrix in a chemo- and site-nonselective manner. Although this platform would seem to run counter to the more typical approach of small-molecule immobilisation on an affinity matrix (i.e., selective coupling), it has been successfully utilised in the past decade to screen protein targets for many bioactive small molecules. This review describes the status of the photo-cross-linking methodology while providing a useful tutorial for academic and industrial chemical biologists who are involved or interested in drug target identification.

Graphical abstract: Photo-cross-linked small-molecule affinity matrix as a tool for target identification of bioactive small molecules

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Publication details

The article was received on 16 Sep 2015 and first published on 24 Feb 2016


Article type: Review Article
DOI: 10.1039/C5NP00117J
Citation: Nat. Prod. Rep., 2016,33, 709-718

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    Photo-cross-linked small-molecule affinity matrix as a tool for target identification of bioactive small molecules

    N. Kanoh, Nat. Prod. Rep., 2016, 33, 709
    DOI: 10.1039/C5NP00117J

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