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Issue 45, 2019
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Tuning the interactions of decavanadate with thaumatin, lysozyme, proteinase K and human serum proteins by its coordination to a pentaaquacobalt(ii) complex cation

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Abstract

The decavanadate anion, HxV10O28(6−x)− (V10), is one of the most studied vanadium polyoxometalate species. In recent decades several works have pointed to its biological relevance coming mainly from its ability to bind to proteins (such as actin, myosin or ion pumps). On the other hand, non-functional binding was observed in several protein crystal structures, where V10 was incorporated “accidentally” resulting from the presence of Na3VO4 as a phosphatase inhibitor. In this work we broaden the potential biological applications of V10 by presenting the synthesis and characterization of two decavanadate species where the anion acts as a ligand: (2-hepH)(NH4)[{Cu(H2O)2(2-hep)}2V10O28]·4H2O (V10Cu) and (2-hepH)2[{Co(H2O)5}2V10O28]·4H2O (V10Co) (2-hep = 2-hydroxyethylpyridine). Unlike free decavanadate, the complex anions stay intact in model buffer solutions (0.1 M 2-(N-morpholino)ethanesulfonic acid, 0.5 M NaCl, pH = 5.8 and 8.0). It has been shown that V10Co is stable also in the presence of proteins and for the first time it was possible to study the interaction of decavanadate with proteins without the interference of lower vanadate oligomers. This allowed comparison of interactions of V10 and V10Co with the model proteins thaumatin, lysozyme, proteinase K, human serum albumin and transferrin under conditions close to biological ones (0.1 M 2-(N-morpholino)ethanesulfonic acid, 0.5 M NaCl, pH = 5.8). The linewidths of the signals at half-height in 51V NMR spectra reflect the strength of interaction of a vanadium species with a protein, and thus it was shown that V10 and V10Co both bind strongly to thaumatin, V10 binds to lysozyme and V10Co binds to proteinase K. V10 interacts with both human serum albumin and transferrin, but surprisingly V10Co exhibits high affinity to transferrin but does not interact with albumin.

Graphical abstract: Tuning the interactions of decavanadate with thaumatin, lysozyme, proteinase K and human serum proteins by its coordination to a pentaaquacobalt(ii) complex cation

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Publication details

The article was received on 14 May 2019, accepted on 14 Oct 2019 and first published on 28 Oct 2019


Article type: Paper
DOI: 10.1039/C9NJ02495F
New J. Chem., 2019,43, 17863-17871
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    Tuning the interactions of decavanadate with thaumatin, lysozyme, proteinase K and human serum proteins by its coordination to a pentaaquacobalt(II) complex cation

    L. Krivosudský, A. Roller and A. Rompel, New J. Chem., 2019, 43, 17863
    DOI: 10.1039/C9NJ02495F

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