Issue 17, 2019

Constructing a far-red to near-infrared fluorescent probe for highly specific detection of cysteine and its bioimaging applications in living cells and zebrafish

Abstract

Cys is a common and important sulfur-containing amino acid in living organisms, whose intracellular level changes are associated with a variety of diseases. To specifically detect Cys without interference from other thiol species has considerable significance. However, only selective fluorescent probes are available for cysteine detection; other thiol species can also have certain interference in the sensing process. Herein, we report an irreversible fluorescent probe for the specific detection of cysteine without interference from other thiol species both in living cells and zebrafish. The FR-NIR-Cys probe exhibits a large emission wavelength shift (up to 84 nm) and a significant ratio fluorescence enhancement (up to 41-fold) in the far-red and near-infrared region. Furthermore, we demonstrate that FR-NIR-Cys with good cell permeability can be employed in the far-red to near-infrared imaging of Cys in living cells and zebrafish.

Graphical abstract: Constructing a far-red to near-infrared fluorescent probe for highly specific detection of cysteine and its bioimaging applications in living cells and zebrafish

Supplementary files

Article information

Article type
Paper
Submitted
16 Jan 2019
Accepted
26 Mar 2019
First published
27 Mar 2019

New J. Chem., 2019,43, 6696-6701

Constructing a far-red to near-infrared fluorescent probe for highly specific detection of cysteine and its bioimaging applications in living cells and zebrafish

S. Zhang, F. Cai, B. Hou, H. Chen, C. Gao, X. Shen and H. Liang, New J. Chem., 2019, 43, 6696 DOI: 10.1039/C9NJ00260J

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