Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 19, 2018
Previous Article Next Article

Ultrasensitive and highly selective FRET aptasensor for Hg2+ measurement in fish samples using carbon dots/AuNPs as donor/acceptor platform

Author affiliations

Abstract

In this study, a novel fluorescence resonance energy transfer (FRET) aptasensor for detection of Hg2+ ions in real samples is described. The sensor is based on single-stranded DNA (ssDNA)-modified carbon dots (CDs) as an energy donor and complementary DNA (cDNA)-modified Au nanoparticles (AuNPs) as an energy acceptor. The CDs were synthesized by the hydrothermal reaction of histidine. The resulting CDs were conjugated with ssDNA through amidation reaction. The AuNPs-cDNA sample was prepared by self-immobilization of thiolated cDNA on the surface of Au nanoparticles. Specific hybridization between ssDNA and cDNA was achieved by mixing CDs-ssDNA and AuNPs-cDNA. The result of this phenomenon is quenching of the fluorescence associated with the FRET process. The response of the aptasensor was based on selective interaction of Hg2+ ions with thymine (T) groups in CDs-ssDNA, which led to displacement of AuNPs-cDNA by Hg2+ and an increase in fluorescence intensity. The aptasensor could be used in the range from 1.3 × 10−12 to 2.4 × 10−5 M for Hg2+ ions with a detection limit of 7.5 × 10−13 M. It showed high selectivity for Hg2+ with respect to several common metal ions. This aptasensor has been used for the determination of mercury ion concentration in fish samples.

Graphical abstract: Ultrasensitive and highly selective FRET aptasensor for Hg2+ measurement in fish samples using carbon dots/AuNPs as donor/acceptor platform

Back to tab navigation

Publication details

The article was received on 06 Jun 2018, accepted on 17 Aug 2018 and first published on 22 Aug 2018


Article type: Paper
DOI: 10.1039/C8NJ02781A
Citation: New J. Chem., 2018,42, 16027-16035

  •   Request permissions

    Ultrasensitive and highly selective FRET aptasensor for Hg2+ measurement in fish samples using carbon dots/AuNPs as donor/acceptor platform

    S. Amiri, R. Ahmadi, A. Salimi, A. Navaee, S. Hamd Qaddare and M. K. Amini, New J. Chem., 2018, 42, 16027
    DOI: 10.1039/C8NJ02781A

Search articles by author

Spotlight

Advertisements