Issue 11, 2018

Mesoporous polymeric microspheres with high affinity for phosphorylated biomolecules

Abstract

Phosphate plays a central role in the environment and in biology. This has led to the demand for a specific phosphate receptor that can be used to sense, enrich or separate phosphate and phosphorylated molecules from complex mixtures. Here, we report an approach for the production of phospho-affinity porous beads with controllable size and pore structure leading to significantly improved chromatographic properties. The beads were prepared by polymerization of bis-imidazolium based host monomer and crosslinker inside the pores of macroporous silica beads that post-etching resulted in mesoporous polymer replicas. The silica precursor, silica–polymer composite and porous polymer replica were characterized by using SEM, IR spectroscopy, optical microscopy and thermogravimetric analysis. Bis-imidazolium functionalized materials were capable of binding and enriching the signaling lipid sphingosine-1-phosphate from plasma samples. Moreover, these engineered materials combined with artificial receptors can recognize phosphorylated amino acids/peptides and are applicable to selective recognition of a broad class of phosphorylated biomolecules.

Graphical abstract: Mesoporous polymeric microspheres with high affinity for phosphorylated biomolecules

Supplementary files

Article information

Article type
Paper
Submitted
07 Mar 2018
Accepted
31 Mar 2018
First published
02 Apr 2018

New J. Chem., 2018,42, 8603-8608

Mesoporous polymeric microspheres with high affinity for phosphorylated biomolecules

S. Shinde, J. Selvalatchmanan, A. Incel, M. Akhoundian, A. K. Bendt and F. Torta, New J. Chem., 2018, 42, 8603 DOI: 10.1039/C8NJ01114A

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