Sensitive and label-free fluorescence detection of apurinic/apyrimidinic endonuclease 1 activity based on isothermal amplified-generation of G-quadruplex

Abstract

A label-free fluorescence assay for the detection of apurinic/apyrimidinic endonuclease 1 activity was developed based on polymerase and nicking endonuclease co-assisted isothermal amplification and G-quadruplex/ligand interaction. Through signal amplification, this method displays high sensitivity with a detection limit of 0.006 U mL⁻¹.