Synthesis, structure, spectroscopy of four novel supramolecular complexes and cytotoxicity study by application of multiple parallel perfused microbioreactors

Abstract

Four novel supramolecular complexes [Cd(H₂L)₂]·(NO₃)₂·2H₂O (1), [Cd(H₂L)₂]·(OH)₂·(EtOH)_0.5 (2), [Zn(H₂L)(2,6-pdc)]·(2,6-H₂pdc)_1.5·MeOH·0.5H₂O (3) and [Zn(H₂L)₂][Zn(2,5-Hpdc)₃]₂·1.5H₂O (4) (H₂L = 2,6-di-(5-methyl-1H-pyrazol-3-yl)pyridine, 2,6-H₂pdc = 2,6-pyridinedicarboxylic acid, 2,5-H₂pdc = 2,5-pyridinedicarboxylic acid) based on pyrazolyl derivatives as ligands were designed and synthesized. They were characterized by elemental analysis, IR and UV-vis spectroscopy, powder X-ray diffraction and single-crystal X-ray diffraction. The structural analysis indicates that the central metal atom (cadmium and zinc atoms) is six-coordinated, and possesses a slightly distorted-octahedral configuration. In addition, a cytotoxicity study was performed using multiple parallel perfused microbioreactors and the potential apoptotic effects on adrenal pheochromocytoma cells and human adipose tissue-derived stem cells were examined by flow-cytometric analysis. The results show that the percentage of apoptotic cells becomes gradually higher as the concentration of the supramolecular complex is increased.