Jump to main content
Jump to site search

Issue 6, 2019

Bacterial flagella as an osteogenic differentiation nano-promoter

Author affiliations

Abstract

Flagella as protein nanofibers (∼14 nm wide) on the surface of swimming bacteria are molecular machines for assisting bacteria to swim in the liquid. They are mainly assembled from protein subunits (FliC) that can be genetically engineered to display peptides. However, so far, no study has been made to show whether flagella with or without displaying peptides could direct stem cell fate. Here we show that flagella detached from bacteria could promote the osteogenic differentiation of bone marrow derived mesenchymal stem cells (BMSCs), and the display of a functional peptide and mineralization of bone mineral (hydroxylapatite, HAP) on the flagella further collectively enhance the promotion effect. The functional peptide is made of two fused amino acid sequences, RGD and E8, which are responsible for promoting cell adhesion onto flagella-bearing substrates and inducing HAP mineralization on flagella from an HAP-supersaturated solution, respectively. Our work shows that the unique nanotopography and surface chemistry of both mineralized and non-mineralized flagella enable them to present physical and chemical cues favoring the osteogenic differentiation of stem cells. Thus flagella are nanofibrous osteogenic differentiation promoters that can be used to build extracellular matrix-like materials.

Graphical abstract: Bacterial flagella as an osteogenic differentiation nano-promoter

Supplementary files

Article information


Submitted
26 Feb 2019
Accepted
07 May 2019
First published
12 Jun 2019

Nanoscale Horiz., 2019,4, 1286-1292
Article type
Communication
Author version available

Bacterial flagella as an osteogenic differentiation nano-promoter

D. Li, Y. Zhu, T. Yang, M. Yang and C. Mao, Nanoscale Horiz., 2019, 4, 1286 DOI: 10.1039/C9NH00124G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements