Jump to main content
Jump to site search

Issue 9, 2020

Polycationic gold nanorods as multipurpose in vitro microtubule markers

Author affiliations

Abstract

Gold nanoparticles are intriguing because of their unique size- and shape-dependent chemical, electronic and optical properties. Gold nanorods (AuNRs) are particularly promising for various sensor applications due to their tip-enhanced plasmonic fields. For biomolecule attachment, AuNRs are often functionalized with proteins. However, by their intrinsic size such molecules block the most sensitive near-field region of the AuNRs. Here, we used short cationic thiols to functionalize AuNRs. We show that the functionalization layer is thin and that these polycationic AuNRs bind in vitro to negatively charged microtubules. Furthermore, we can plasmonically stimulate light emission from single AuNRs in the absence of any fluorophores and, therefore, use them as bleach- and blinkfree microtubule markers. We expect that polycationic AuNRs may be applicable to in vivo systems and other negatively charged molecules like DNA. In the long-term, microtubule-bound AuNRs can be used as ultrasensitive single-molecule sensors for molecular machines that interact with microtubules.

Graphical abstract: Polycationic gold nanorods as multipurpose in vitro microtubule markers

Article information


Submitted
20 May 2020
Accepted
12 Jul 2020
First published
13 Jul 2020

This article is Open Access

Nanoscale Adv., 2020,2, 4003-4010
Article type
Paper

Polycationic gold nanorods as multipurpose in vitro microtubule markers

V. Wedler, F. Strauß, S. Sudhakar, G. L. Hermsdorf, Y. Stierhof and E. Schäffer, Nanoscale Adv., 2020, 2, 4003 DOI: 10.1039/D0NA00406E

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements