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Issue 8, 2019
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A MoS2–MWCNT based fluorometric nanosensor for exosome detection and quantification

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Abstract

Circulating exosomes in body fluids are involved in many diseases and have important roles in pathophysiological processes. Specifically, they have emerged as a promising new class of biomarkers in cancer diagnosis and prognosis because of their high concentration and availability in a variety of biological fluids. The ability to quantitatively detect and characterize these nano-sized vesicles is crucial to make use of exosomes as a reliable biomarker for clinical applications. However, current methods are mostly technically challenging and time-consuming which prevents them from being adopted in clinical practice. In this work, we have developed a rapid sensitive platform for exosome detection and quantification by employing MoS2–multiwall carbon nanotubes as a fluorescence quenching material. This exosome biosensor shows a sensitive and selective biomarker detection. Using this MoS2–MWCNT based fluorometric nanosensor to analyze exosomes derived from MCF-7 breast cancer cells, we found that CD63 expression could be measured based on the retrieved fluorescence of the fluorophore with a good linear response range of 0–15% v/v. In addition, this nanosensing technique is able to quantify exosomes with different surface biomarker expressions and has revealed that exosomes secreted from MCF-7 breast cancer cells have a higher CD24 expression compared to CD63 and CD81.

Graphical abstract: A MoS2–MWCNT based fluorometric nanosensor for exosome detection and quantification

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Supplementary files

Article information


Submitted
16 Apr 2019
Accepted
18 Jun 2019
First published
19 Jun 2019

This article is Open Access

Nanoscale Adv., 2019,1, 2866-2872
Article type
Paper

A MoS2–MWCNT based fluorometric nanosensor for exosome detection and quantification

M. Tayebi, M. Tavakkoli Yaraki, H. Y. Yang and Y. Ai, Nanoscale Adv., 2019, 1, 2866
DOI: 10.1039/C9NA00248K

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    [Original citation] - Published by The Royal Society of Chemistry.

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