Issue 8, 2019
From the journal:

Nanoscale Advances

A novel method for genetic transformation of C. albicans using modified-hydroxyapatite nanoparticles as a plasmid DNA vehicle

Ketaki Deshmukh,†a   Sutapa Roy Ramanan ORCID logo †*b  and  Meenal Kowshik ORCID logo †*a  

* Corresponding authors

a Biological Sciences Department, Birla Institute of Technology and Science Pilani, K K Birla Goa Campus, Goa, India
E-mail: ketaki03@gmail.com, meenal@goa.bits-pilani.ac.in

b Chemical Engineering Department, Birla Institute of Technology and Science Pilani, K K Birla Goa Campus, Goa, India
E-mail: sutapa@goa.bits-pilani.ac.in

Abstract

In modern biological research, genetic transformation is an important molecular biology technique with extensive applications. In this work, we describe a new method for the delivery of plasmid DNA (pDNA) into a yeast species, Candida albicans. This method is based on the use of novel arginine–glucose–PEG functionalized hydroxyapatite nanoparticles (M-HAp NPs) as a vehicle which delivers pDNA into Candida albicans with a high transformation efficiency of 106 cfu μg−1 of pDNA, without the need for preparation of competent cells. A four-fold higher transformation efficiency as compared to that of the electroporation method was obtained. This new method could provide exciting opportunities for the advancement of the applications of yeasts in the field of biotechnology.

Graphical abstract: A novel method for genetic transformation of C. albicans using modified-hydroxyapatite nanoparticles as a plasmid DNA vehicle

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Article information

DOI
https://doi.org/10.1039/C8NA00365C
Article type
Paper
Submitted
25 Nov 2018
Accepted
10 Jun 2019
First published
11 Jun 2019
This article is Open Access
Creative Commons BY-NC license

Nanoscale Adv., 2019,1, 3015-3022

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A novel method for genetic transformation of C. albicans using modified-hydroxyapatite nanoparticles as a plasmid DNA vehicle

K. Deshmukh, S. R. Ramanan and M. Kowshik, Nanoscale Adv., 2019, 1, 3015 DOI: 10.1039/C8NA00365C

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