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Issue 1, 2018
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Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite

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Abstract

Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has become a powerful and fast tool to evaluate the elemental composition at a single-cell level. In this study, the cellular bioavailability of arsenite (incubation of 25 and 50 μM for 0–48 h) has been successfully assessed by SC-ICP-MS/MS for the first time directly after re-suspending the cells in water. This procedure avoids the normally arising cell membrane permeabilization caused by cell fixation methods (e.g. methanol fixation). The reliability and feasibility of this SC-ICP-MS/MS approach with a limit of detection of 0.35 fg per cell was validated by conventional bulk ICP-MS/MS analysis after cell digestion and parallel measurement of sulfur and phosphorus.

Graphical abstract: Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite

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Supplementary files

Article information


Submitted
09 Oct 2017
Accepted
13 Dec 2017
First published
13 Dec 2017

This article is Open Access

Metallomics, 2018,10, 73-76
Article type
Communication

Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite

S. Meyer, A. López-Serrano, H. Mitze, N. Jakubowski and T. Schwerdtle, Metallomics, 2018, 10, 73
DOI: 10.1039/C7MT00285H

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