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Issue 10, 2017
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Differences in cisplatin distribution in sensitive and resistant ovarian cancer cells: a TEM/NanoSIMS study

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Abstract

Cisplatin is a widely used anti-cancer drug, but its effect is often limited by acquired resistance to the compound during treatment. Here, we use a combination of transmission electron microscopy (TEM) and nanoscale-secondary ion mass spectrometry (NanoSIMS) to reveal differences between cisplatin uptake in human ovarian cancers cells, which are known to be susceptible to acquired resistance to cisplatin. Both cisplatin sensitive and resistant cell lines were studied, revealing markedly less cisplatin in the resistant cell line. In cisplatin sensitive cells, Pt was seen to distribute diffusely in the cells with hotspots in the nucleolus, mitochondria, and autophagosomes. Inductively coupled plasma mass spectrometry (ICP-MS) was used to validate the NanoSIMS results.

Graphical abstract: Differences in cisplatin distribution in sensitive and resistant ovarian cancer cells: a TEM/NanoSIMS study

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Article information


Submitted
11 May 2017
Accepted
07 Sep 2017
First published
07 Sep 2017

This article is Open Access

Metallomics, 2017,9, 1413-1420
Article type
Paper

Differences in cisplatin distribution in sensitive and resistant ovarian cancer cells: a TEM/NanoSIMS study

R. F. S. Lee, T. Riedel, S. Escrig, C. Maclachlan, G. W. Knott, C. A. Davey, K. Johnsson, A. Meibom and P. J. Dyson, Metallomics, 2017, 9, 1413
DOI: 10.1039/C7MT00153C

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