Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 22nd May 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 1, 2013
Previous Article Next Article

Subcellular redistribution and mitotic inheritance of transition metals in proliferating mouse fibroblast cells

Author affiliations

Abstract

Synchrotron X-ray fluorescence microscopy of non-synchronized NIH 3T3 fibroblasts revealed an intriguing redistribution dynamics that defines the inheritance of trace metals during mitosis. At metaphase, the highest density areas of Zn and Cu are localized in two distinct regions adjacent to the metaphase plate. As the sister chromatids are pulled towards the spindle poles during anaphase, Zn and Cu gradually move to the center and partition into the daughter cells to yield a pair of twin pools during cytokinesis. Colocalization analyses demonstrated high spatial correlations between Zn, Cu, and S throughout all mitotic stages, while Fe showed consistently different topographies characterized by high-density spots distributed across the entire cell. Whereas the total amount of Cu remained similar compared to interphase cells, mitotic Zn levels increased almost 3-fold, suggesting a prominent physiological role that lies beyond the requirement of Zn as a cofactor in metalloproteins or messenger in signaling pathways.

Graphical abstract: Subcellular redistribution and mitotic inheritance of transition metals in proliferating mouse fibroblast cells

Back to tab navigation

Publication details

The article was received on 03 Sep 2012, accepted on 14 Nov 2012 and first published on 04 Dec 2012


Article type: Paper
DOI: 10.1039/C2MT20176C
Metallomics, 2013,5, 52-61

  •   Request permissions

    Subcellular redistribution and mitotic inheritance of transition metals in proliferating mouse fibroblast cells

    R. McRae, B. Lai and C. J. Fahrni, Metallomics, 2013, 5, 52
    DOI: 10.1039/C2MT20176C

Search articles by author

Spotlight

Advertisements