Jump to main content
Jump to site search

Issue 1, 2013
Previous Article Next Article

A mast cell degranulation screening assay for the identification of novel mast cell activating agents

Author affiliations

Abstract

The development and use of vaccines and their ability to prevent infection/disease is a shining example of the benefit of biomedical research. Modern vaccines often utilize subunit immunogens that exhibit minimal immunogenicity and require the use of adjuvants to maximize the induction of protective immune responses. We recently described a novel class of vaccine adjuvants, mast cell (MC) activators, that exhibit safe and effective vaccine adjuvant activity when administered by intranasal or intradermal routes. A compound library containing 580 functionalized benzopyrans, a structural motif found in a diverse array of natural and designed bioactive compounds, was screened using a MC degranulation assay to identify novel MC activating compounds for future evaluation as novel vaccine adjuvants. This approach identified 12 novel MC degranulating compounds. Therefore, MC degranulation can be used to reliably detect novel compounds for evaluation as adjuvants for use in mucosal vaccine strategies.

Graphical abstract: A mast cell degranulation screening assay for the identification of novel mast cell activating agents

  • This article is part of the themed collection: New Talent
Back to tab navigation

Supplementary files

Publication details

The article was received on 19 Mar 2012, accepted on 06 May 2012 and first published on 21 Jun 2012


Article type: Concise Article
DOI: 10.1039/C2MD20073B
Med. Chem. Commun., 2013,4, 88-94

  •   Request permissions

    A mast cell degranulation screening assay for the identification of novel mast cell activating agents

    H. F. Staats, S. M. Kirwan, H. W. Choi, C. P. Shelburne, S. N. Abraham, G. Y. C. Leung and D. Y.-K. Chen, Med. Chem. Commun., 2013, 4, 88
    DOI: 10.1039/C2MD20073B

Search articles by author

Spotlight

Advertisements