Issue 12, 2014

Alterations in rat pulmonary phosphatidylcholines after chronic exposure to ambient fine particulate matter

Abstract

This study elucidated the underlying pathophysiological changes that occur after chronic ambient fine particulate matter (PM2.5) exposure via a lipidomic approach. Five male Sprague-Dawley rats were continually whole-body exposed to ambient air containing PM2.5 at 16.7 ± 10.1 μg m−3 from the outside of the building for 8 months, whereas a control group (n = 5) inhaled filtered air. Phosphorylcholine-containing lipids were extracted from lung tissue and profiled using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The phosphatidylcholine (PC) signal features of the two groups were compared using partial least squares discriminant analysis (PLS-DA) and Wilcoxon rank sum tests. The PC profile of the exposure group differed from that of the control group; the R2Y and Q2 were 0.953 and 0.677, respectively, in the PLS-DA model. In the exposure group, a significant 0.66- to 0.80-fold reduction in lyso-PC levels, which may have resulted from repeated inflammation, was observed. Decreased surfactant PCs by 16% at most may indicate injuries to alveolar type II cells. Cell function and cell signalling are likely to be altered because the decrease in unsaturated PCs may reduce membrane fluidity. Accompanied by the decline in plasmenylcholines, decreased unsaturated PCs may indicate the attack of reactive oxygen species generated by PM2.5 exposure. The physiological findings conformed to the histopathological changes in the exposed animals. PC profiling using UPLC-MS/MS-based lipidomics is sensitive for reflecting pathophysiological perturbations in the lung after long-term and low concentration PM2.5 exposure.

Graphical abstract: Alterations in rat pulmonary phosphatidylcholines after chronic exposure to ambient fine particulate matter

Article information

Article type
Paper
Submitted
24 Jul 2014
Accepted
11 Sep 2014
First published
11 Sep 2014

Mol. BioSyst., 2014,10, 3163-3169

Author version available

Alterations in rat pulmonary phosphatidylcholines after chronic exposure to ambient fine particulate matter

W. Chen, C. Lin, Y. Yan, K. T. Cheng and T. Cheng, Mol. BioSyst., 2014, 10, 3163 DOI: 10.1039/C4MB00435C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements