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Issue 3, 2013
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Fluorogenic label to quantify the cytosolic delivery of macromolecules

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Abstract

The delivery of a macromolecule to the cytosol of human cells is assessed by using a pendant di-O-glycosylated derivative of fluorescein. Its fluorescence is unmasked by Escherichia coli β-galactosidase installed in the cytosol. Background is diminished by using RNAi to suppress the expression of GLB1, which encodes a lysosomal β-galactosidase. This strategy was used to quantify the cytosolic entry of a highly cationic protein, ribonuclease A.

Graphical abstract: Fluorogenic label to quantify the cytosolic delivery of macromolecules

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Publication details

The article was received on 04 Dec 2012, accepted on 10 Jan 2013 and first published on 10 Jan 2013


Article type: Communication
DOI: 10.1039/C3MB25552B
Mol. BioSyst., 2013,9, 339-342

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    Fluorogenic label to quantify the cytosolic delivery of macromolecules

    T. Chao and R. T. Raines, Mol. BioSyst., 2013, 9, 339
    DOI: 10.1039/C3MB25552B

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